Establishment of a cell culture model based on primary epithelial cells to investigate damage and repair of respiratory epithelial cells
نویسندگان
چکیده
Background The nasal epithelium represents the first barrier against entry of airborne particles into the respiratory system and therefore protects against allergens, pollutants and pathogens. We have previously used the respiratory epithelial cell line 16HBE14oto define factors which can impair the barrier function of the respiratory mucosa. However, cultures of primary epithelial cells obtained from human nasal biopsies should resemble the natural situation in the nose better. Here we investigated primary human nasal epithelial cells regarding growth characteristics and sensitivity to damage by interferon-gamma and other factors.
منابع مشابه
Morphology and Ultrastructure of Mouse Polarized Endometrial Epithelial Cell Monolyer in Vitro
Purpose: The objective for this study is to investigate the morphology and ultrastructure of mouse endometrial epithelial cell monolayer cultured on matrigel in dual-chambered system as an in vitro mouse endometrial epithelial cell culture model that mimics structural and functional properties of the endometrial epithelium in vivo. Materials and Methods: Mouse endometrial epithelial cells were...
متن کاملEffects of low dose radiation on the expression of proteins related to DNA repair requiring Caveolin-1 in human mammary epithelial cells
Background: Radiotherapy is an effective and important therapeutic method for breast cancer, but at the same time it has a radiation-induced bystander effect on normal tissue around the tumor. Repair of double-strand breaks (DSBs) by normal cells can reduce the extent of damage caused by this effect. Caveolin-1 (Cav-1) is an important regulatory molecule in cell signal transduction. However, th...
متن کاملUlcert Repair by Spray of Epithelial Stem Cells
Purpose: Separation, prolifration of stem cells and repairing of injured parts using these cells. Materials and Methods: This was a Lab-Experimental study. We used 6 male albino rabbits. At first, under general anesthesia 5x5 cm2 full thickness skin from one of the rabbits separated, washed using 70% alcohol and inserted in cold HBSS. Then it was cut into 4-6 mm pieces, washed again and incubat...
متن کاملEstablishment of a Primary Cell Culture of Human Fibroblast in Iran
Background: Human fibroblasts are the part of the dermis that secrete extracellular matrix for the purpose of tissue repair. Culturing fibroblasts, which leads to formation of a monolayer of these cells, is used for treating various conditions including thermal burns and other skin defects such as diabetic and varicose vein leg ulcers. Therefore, we aimed at developing a fibroblast bank to acco...
متن کاملModulation of cellular transport characteristics of the human lung alveolar epithelia
Among the drug delivery and targeting (DDT) routes, lung alveolar epithelium has been given enormous attentions in terms of the delivery of a wide range of macromolecules such as gene- or protein-based nanopharmaceuticals. However, little is known about cellular modulation of lung transport characteristics by endogenous and/or exogenous agents. Thus, in the current study, impact of dexamethason...
متن کامل